Anticorps Polyclonal de lapin anti-SMN

• Phare
• Validé par KD/KO

SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 11708-1-PBS

Synonymes

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

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Galerie de données de validation

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  WB analysis of HeLa using 11708-1-AP (same clone as 11708-1-PBS)

  WB result of SMN antibody (11708-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  WB analysis using 11708-1-AP (same clone as 11708-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11708-1-PBS

  K-562 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11708-1-AP (same clone as 11708-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 11708-1-AP (same clone as 11708-1-PBS)

  IP result of anti-SMN (IP:11708-1-AP, 4ug; Detection:11708-1-AP 1:2000) with HEK-293 cells lysate 1040 ug. This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11708-1-AP (SMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 11708-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 11708-1-AP (same clone as 11708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11708-1-AP (same clone as 11708-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (11708-1-AP) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11708-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11708-1-PBS cible SMN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMN Protéine recombinante Ag2260
• Nom complet: survival of motor neuron 2, centromeric
• Masse moléculaire calculée: 282 aa, 30 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000908
• Symbole du gène: SMN
• Identification du gène (NCBI): 6607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.

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