Anticorps Monoclonal anti-SMN-Exon7

SMN-Exon7 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A8G11

N° de cat : 60255-1-PBS

Synonymes

SMN, 3A8G11, Component of gems 1, Gemin 1, Gemin-1

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Galerie de données de validation

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  WB analysis of HEK-293 using 60255-1-Ig (same clone as 60255-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60255-1-Ig (same clone as 60255-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60255-1-Ig (SMN-Exon7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60255-1-Ig (SMN-Exon7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 60255-1-Ig (same clone as 60255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SMN-Exon7 antibody (60255-1-Ig, Clone: 3A8G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60255-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60255-1-PBS cible SMN-Exon7 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMN-Exon7 Protéine recombinante Ag16615
• Nom complet: survival of motor neuron 1, telomeric
• Masse moléculaire calculée: 294 aa, 32 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC062723
• Symbole du gène: SMN
• Identification du gène (NCBI): 6606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7.

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