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Anticorps Polyclonal de lapin anti-SMN

SMN Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 22329-1-AP

Synonymes

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

Galerie de données de validation

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  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    WB result of SMN antibody (22329-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HEK-293 cells.

  • Western Blot (WB) analysis of K-562 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of K-562 using 22329-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HepG2 using 22329-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of HEK-293 using 22329-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SMN Polyclonal antibody (22329-1-AP)

    WB analysis of Jurkat using 22329-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22329-1-AP (SMN antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SMN Polyclonal antibody (22329-1-AP)

    IHC staining of human testis using 22329-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22329-1-AP (SMN antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SMN Polyclonal antibody (22329-1-AP)

    IF Staining of HepG2 using 22329-1-AP

    Immunofluorescent analysis of HepG2 cells using 22329-1-AP (SMN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules K-562, cellules HEK-293, cellules HepG2, cellules Jurkat
Résultats positifs en IHCtissu testiculaire humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22329-1-AP cible SMN dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SMN Protéine recombinante Ag17798
Nom complet survival of motor neuron 2, centromeric
Masse moléculaire calculée 282 aa, 30 kDa
Poids moléculaire observé 38 kDa
Numéro d’acquisition GenBankBC000908
Symbole du gène SMN2
Identification du gène (NCBI) 6607
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purifié par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

Protocole

Product Specific Protocols
WB protocol for SMN antibody 22329-1-APDownload protocol
IHC protocol for SMN antibody 22329-1-APDownload protocol
IF protocol for SMN antibody 22329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols