Anticorps Recombinant de lapin anti-SMN

SMN Recombinant Antibody for WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251620C4

N° de cat : 86668-2-PBS

Synonymes

C BCD541, Component of gems 1, Gemin 1, Gemin-1, SMN1

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Galerie de données de validation

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  WB analysis using 86668-2-RR (same clone as 86668-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86668-2-RR (SMN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02580-1

  The mean SMN concentration was determined to be 13.66 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02580-1

  Sandwich ELISA standard curve of MP02580-1, Human SMN Recombinant Matched Antibody Pair - PBS only. 86668-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4812. 86668-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  IP experiment of HEK-293 using 86668-2-RR (same clone as 86668-2-PBS)

  IP result of anti-SMN (IP: 86668-2-RR, 4 ug; Detection: 86668-2-RR 1:600) with HEK-293 cells lysate 1200 ug. This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86668-2-RR (same clone as 86668-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86668-2-RR (same clone as 86668-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMN antibody (86668-2-RR, Clone: 251620C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86668-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02580-1

  The mean SMN concentration was determined to be 4.5 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load.

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  Cytometric bead array standard curve of MP02580-1

  Cytometric bead array standard curve of MP02580-1, SMN Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86668-2-PBS. Detection antibody: 86668-1-PBS. Standard: Eg4812. Range: 0.312-40 ng/mL.

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Informations sur le produit

86668-2-PBS cible SMN dans les applications de WB, IF/ICC, IP, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMN Protéine recombinante Eg4812
• Nom complet: survival of motor neuron 2, centromeric
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC015308
• Symbole du gène: SMN
• Identification du gène (NCBI): 6607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

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