Anticorps Polyclonal de lapin anti-SMPD1,ASM

SMPD1,ASM Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 30197-1-AP

Synonymes

SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM

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Galerie de données de validation

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  WB analysis of human placenta using 30197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30197-1-AP (SMPD1,ASM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 30197-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SMPD1,ASM antibody (30197-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30197-1-AP cible SMPD1,ASM dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMPD1,ASM Protéine recombinante Ag32479
• Nom complet: sphingomyelin phosphodiesterase 1, acid lysosomal
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC041164
• Symbole du gène: SMPD1
• Identification du gène (NCBI): 6609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing. As a catalytically inactive 75kDa precursor, after signal peptide cleavage, it was processed into smaller non-glycosylated and rapidly degraded 57kDa form in ER-Golgi complex. And 70kDa mature form with major catalytic activity located in lysosomes, which is further processed into 52kDa form (PMID:25853898, PMID:26499107).

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