Anticorps Monoclonal anti-SMS

• Phare
• Validé par KD/KO

SMS Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1G8E9

Avis (1)

N° de cat : 68040-1-Ig

Synonymes

Spermine synthase, 1G8E9, EC:2.5.1.22, MRSR, SPMSY

---

Galerie de données de validation

• 

  WB analysis using 68040-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of K-562 using 68040-1-Ig

  WB result of SMS antibody (68040-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMS transfected K-562 cells.

• 

  IF Staining of HeLa using 68040-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 68040-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68040-1-Ig cible SMS dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMS Protéine recombinante Ag8840
• Nom complet: spermine synthase
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC009898
• Symbole du gène: SMS
• Identification du gène (NCBI): 6611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hua (Verified Customer) (12-10-2024)

Works with mouse liver on WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver

SMS Antibody Western Blot validation (1:500 dilution) in mouse liver (Cat no:68040-1-Ig)

{{ptg:RelatedPrimaryAntibodies}}