Anticorps Monoclonal anti-SMS

• Phare
• Validé par KD/KO

SMS Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1G8E9

N° de cat : 68040-1-PBS

Synonymes

Spermine synthase, 1G8E9, EC:2.5.1.22, MRSR, SPMSY

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Galerie de données de validation

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  WB analysis using 68040-1-Ig (same clone as 68040-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68040-1-Ig (SMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68040-1-Ig (same clone as 68040-1-PBS)

  WB result of SMS antibody (68040-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMS transfected K-562 cells. This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68040-1-Ig (same clone as 68040-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMS antibody (68040-1-Ig, Clone: 1G8E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68040-1-Ig (same clone as 68040-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SMS (68040-1-Ig, Clone:1G8E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68040-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68040-1-PBS cible SMS dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMS Protéine recombinante Ag8840
• Nom complet: spermine synthase
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC009898
• Symbole du gène: SMS
• Identification du gène (NCBI): 6611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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