Anticorps Recombinant de lapin anti-SMUG1

SMUG1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240817E4

N° de cat : 83771-5-PBS

Synonymes

UNG3, Single-strand selective monofunctional uracil DNA glycosylase, HMUDG, FDG, EC:3.2.2.-

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Galerie de données de validation

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  WB analysis using 83771-5-RR (same clone as 83771-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83771-5-RR (SMUG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 83771-5-RR (same clone as 83771-5-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug SMUG1 Recombinant antibody (83771-5-RR, Clone:240817E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83771-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83771-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83771-5-RR against Human SMUG1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83771-5-PBS cible SMUG1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMUG1 Protéine recombinante Ag13934
• Nom complet: single-strand-selective monofunctional uracil-DNA glycosylase 1
• Masse moléculaire calculée: 177 aa, 20 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000417
• Symbole du gène: SMUG1
• Identification du gène (NCBI): 23583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930).Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

{{ptg:RelatedPrimaryAntibodies}}