Anticorps Polyclonal de lapin anti-SMURF1

• Phare
• Validé par KD/KO

SMURF1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55175-1-PBS

Synonymes

E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1, KIAA1625

---

Galerie de données de validation

• 

  WB analysis using 55175-1-AP (same clone as 55175-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55175-1-AP (SMURF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55175-1-AP (same clone as 55175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55175-1-AP (SMURF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 55175-1-AP (same clone as 55175-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 55175-1-AP (same clone as 55175-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using SMURF1 antibody (55175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55175-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

55175-1-PBS cible SMURF1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD specific E3 ubiquitin protein ligase 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_020429
• Symbole du gène: SMURF1
• Identification du gène (NCBI): 57154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. The antibody is specific to SMURF1. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

{{ptg:RelatedPrimaryAntibodies}}