Anticorps Monoclonal anti-SMURF1

SMURF1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A7E4

N° de cat : 68466-1-PBS

Synonymes

2A7E4, E3 ubiquitin-protein ligase SMURF1, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF1, hSMURF1

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Galerie de données de validation

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  WB analysis using 68466-1-Ig (same clone as 68466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68466-1-Ig (SMURF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68466-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68466-1-PBS cible SMURF1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMURF1 Protéine recombinante Ag30891
• Nom complet: SMAD specific E3 ubiquitin protein ligase 1
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: NM_020429
• Symbole du gène: SMURF1
• Identification du gène (NCBI): 57154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

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