Anticorps Polyclonal de lapin anti-SMURF2

• Phare
• Validé par KD/KO

SMURF2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18038-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

  IP result of anti-SMURF2 (IP:18038-1-AP, 4ug; Detection:18038-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18038-1-PBS cible SMURF2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMURF2 Protéine recombinante Ag12666
• Nom complet: SMAD specific E3 ubiquitin protein ligase 2
• Masse moléculaire calculée: 748 aa, 86 kDa
• Poids moléculaire observé: 86-100 kDa
• Numéro d’acquisition GenBank: BC093876
• Symbole du gène: SMURF2
• Identification du gène (NCBI): 64750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).

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