Anticorps Recombinant de lapin anti-SMURF2

SMURF2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241486F3

N° de cat : 84445-1-PBS

Synonymes

241486F3, E3 ubiquitin-protein ligase SMURF2, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF2, hSMURF2

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Galerie de données de validation

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  IHC staining of mouse lung using 84445-1-RR (same clone as 84445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84445-1-RR (SMURF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84445-1-PBS cible SMURF2 dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMURF2 Protéine recombinante Ag33419
• Nom complet: SMAD specific E3 ubiquitin protein ligase 2
• Masse moléculaire calculée: 748 aa, 86 kDa
• Numéro d’acquisition GenBank: BC093876
• Symbole du gène: SMURF2
• Identification du gène (NCBI): 64750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).

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