Anticorps Polyclonal de lapin anti-SMYD2

• Phare
• Validé par KD/KO

SMYD2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

Avis (1)

N° de cat : 21290-1-AP

Synonymes

HSKM B, KMT3C, Lysine N methyltransferase 3C, SMYD2, ZMYND14

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Galerie de données de validation

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  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21290-1-AP (SMYD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 21290-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21290-1-AP (SMYD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 21290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMYD2 antibody (21290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellule C2C12, cellules C2C12, cellules HEK-293, cellules U2OS, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

21290-1-AP cible SMYD2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMYD2 Protéine recombinante Ag15823
• Nom complet: SET and MYND domain containing 2
• Masse moléculaire calculée: 433 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC098305
• Symbole du gène: SMYD2
• Identification du gène (NCBI): 56950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMYD2, also known as SET and MYND domain containing 2, encodes an enzyme called N-lysine methyltransferase SMYD2, which plays a crucial role in cellular processes, including histone methylation, non-histone protein methylation, cell proliferation and differentiation, DNA repair (PMID: 18065756, PMID: 36191822). Aberrant SMYD2 expression has been linked to various cancers, including colon cancer, lung cancer, and cervical cancer. Smyd2 is strongly expressed in muscle cells with highest expression in the neonatal heart (PMID: 25125178).

Publications

Species	Application	Title
mouse	IHC	Cell Death Dis; SMYD2 targets RIPK1 and restricts TNF-induced apoptosis and necroptosis to support colon tumor growth.; Authors - Yu-Qiang Yu
human	IHC	Theranostics; Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma.; Authors - Libin Yan ; KD Validated
human	WB	Stem Cells; SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells through Mediating the Transcriptional Activation of Key Mesendodermal Genes.; Authors - Hua-Jun Bai ; KO Validated
human	WB,IHC	Am J Cancer Res; SMYD2 suppresses APC2 expression to activate the Wnt/β-catenin pathway and promotes epithelial-mesenchymal transition in colorectal cancer.; Authors - Fanchao Meng ; KD Validated
mouse	WB,IHC,IF	J Pharmacol Sci; Pharmacological inhibition of SMYD2 protects against cisplatin-induced renal fibrosis and inflammation; Authors - Min Chen
human	WB	Exp Cell Res; SMYD2 suppresses p53 activity to promote glucose metabolism in cervical cancer.; Authors - Ying Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deyong (Verified Customer) (03-11-2022)

This antibody worked well in the differentiated human cardiomyocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human cardiomyocytes