Anticorps Monoclonal anti-SMYD3

SMYD3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B5C10

N° de cat : 66330-1-PBS

Synonymes

SMYD3, ZMYND1, ZNFN3A1

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Galerie de données de validation

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  WB analysis of A431 using 66330-1-Ig (same clone as 66330-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66330-1-Ig (same clone as 66330-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66330-1-Ig (same clone as 66330-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SMYD3 antibody (66330-1-Ig, Clone: 1B5C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66330-1-PBS cible SMYD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMYD3 Protéine recombinante Ag2624
• Nom complet: SET and MYND domain containing 3
• Masse moléculaire calculée: 428aa,49 kDa; 369aa,42 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC031010
• Symbole du gène: SMYD3
• Identification du gène (NCBI): 64754
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).

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