Anticorps Polyclonal de lapin anti-SNAI1

• Phare
• Validé par KD/KO

SNAI1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

370

Avis (2)

N° de cat : 13099-1-AP

Synonymes

Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

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Galerie de données de validation

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  WB analysis of mouse heart using 13099-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13099-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13099-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13099-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 13099-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13099-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 13099-1-AP

  IP Result of anti-SNAI1 (IP:13099-1-AP, 4ug; Detection:13099-1-AP 1:600) with MCF-7 cells lysate 1040ug.

• 

  IHC staining of human stomach cancer using 13099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13099-1-AP (SNAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13099-1-AP (SNAI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13099-1-AP (SNAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules BxPC-3, cellules COLO 320, cellules MCF-7, cellules PC-3, tissu cardiaque humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 340 publications below
• IHC: See 1 publications below
• IF: See 29 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

13099-1-AP cible SNAI1 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAI1 Protéine recombinante Ag3723
• Nom complet: snail homolog 1 (Drosophila)
• Masse moléculaire calculée: 264 aa, 29 kDa
• Poids moléculaire observé: 29-35 kDa
• Numéro d’acquisition GenBank: BC012910
• Symbole du gène: SNAI1
• Identification du gène (NCBI): 6615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Publications

Species	Application	Title
mouse		Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Biomater Res; Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA.; Authors - Chunping Yu
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
human	WB	Cancer Res; An in vivo CRISPR screen identifies that SNRPC promotes triple-negative breast cancer progression; Authors - Xun-Xi Lu
human	WB	Autophagy; TGFB1-induced autophagy affects the pattern of pancreatic cancer progression in distinct ways depending on SMAD4 status.; Authors - Chen Liang
human	WB	Aging Cell; Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation.; Authors - Liling Xiong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:2000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells

FH

Hala (Verified Customer) (11-23-2021)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2

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