Anticorps Recombinant de lapin anti-SNAI1

SNAI1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240164C2

N° de cat : 81584-4-PBS

Synonymes

240164C2, SLUGH 2, SLUGH2, SNAI 1, SNAIL1

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00343-2

  The mean SNAI1 concentration was determined to be 3,819.6 pg/mL in HCT116 cell extract based on a 3.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00343-2

  Sandwich ELISA standard curve of MP00343-2, Human SNAI1 Recombinant Matched Antibody Pair - PBS only. 81584-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24248. 81584-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-1000 pg/mL.

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  IF Staining of HeLa using 81584-4-RR (same clone as 81584-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNAI1 antibody (81584-4-RR, Clone: 240164C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 81584-4-RR (same clone as 81584-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-4-RR, Clone:240164C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81584-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00343-2

  Cytometric bead array standard curve of MP00343-2, SNAI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81584-4-PBS. Detection antibody: 81584-3-PBS. Standard: Ag24248. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 81584-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81584-4-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81584-4-PBS cible SNAI1 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNAI1 Protéine recombinante Ag24248
• Nom complet: snail homolog 1 (Drosophila)
• Masse moléculaire calculée: 264 aa, 29 kDa
• Numéro d’acquisition GenBank: BC012910
• Symbole du gène: SNAI1
• Identification du gène (NCBI): 6615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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