Anticorps Recombinant de lapin anti-SNAI1

SNAI1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240164C11

N° de cat : 81584-5-RR

Synonymes

Zinc finger protein SNAI1, SNAIL1, SNAI 1, SLUGH2, SLUGH 2

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Galerie de données de validation

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  WB analysis using 81584-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81584-5-RR (SNAI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 81584-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SNAI1 Recombinant antibody (81584-5-RR, Clone:240164C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 81584-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81584-5-RR against Human SNAI1 were performed. The affinity constant is 0.64 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules BxPC-3, cellules MCF-7
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81584-5-RR cible SNAI1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNAI1 Protéine recombinante Ag24248
• Nom complet: snail homolog 1 (Drosophila)
• Masse moléculaire calculée: 264 aa, 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012910
• Symbole du gène: SNAI1
• Identification du gène (NCBI): 6615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

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