Anticorps Polyclonal de lapin anti-SNAI2/SLUG

SNAI2/SLUG Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC (Intra), CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(194)

Avis (1)

N° de cat : 12129-1-AP

Synonymes

SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1

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Galerie de données de validation

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12129-1-AP (SNAI2/SLUG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 12129-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12129-1-AP (SNAI2/SLUG antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 12129-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human SNAI2 (12129-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu cardiaque de rat, tissu cérébral de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 175 publications below
• IHC: See 21 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12129-1-AP cible SNAI2/SLUG dans les applications de WB, IHC, IF, FC (Intra), CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAI2/SLUG Protéine recombinante Ag2773
• Nom complet: snail homolog 2 (Drosophila)
• Masse moléculaire calculée: 268 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015895
• Symbole du gène: SLUG
• Identification du gène (NCBI): 6591
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	J Exp Clin Cancer Res; Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer; Authors - Li Zhang
human	WB	Cell Death Dis; UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer; Authors - Xiaoling Hong
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan
mouse	WB,IF	Theranostics; Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.; Authors - Shuyu Guo
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:500

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Glioblastoma cells

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