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Anticorps Polyclonal de lapin anti-SNAP25

SNAP25 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

, ELISA

Conjugaison

Non conjugué

N° de cat : 10007-1-AP

Synonymes

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

Galerie de données de validation

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  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10007-1-AP cible SNAP25 dans les applications de , ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Protéine recombinante
Nom complet synaptosomal-associated protein, 25kDa
Masse moléculaire calculée 23 kDa
Numéro d’acquisition GenBankBC010647
Symbole du gène SNAP25
Identification du gène (NCBI) 6616
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Protocole

Product Specific Protocols
WB protocol for SNAP25 antibody 10007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols