Anticorps Polyclonal de lapin anti-SNAP25

• Phare
• Validé par KD/KO

SNAP25 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (2)

N° de cat : 14903-1-AP

Synonymes

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 14903-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14903-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14903-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14903-1-AP

  IP Result of anti-SNAP25 (IP:14903-1-AP, 3ug; Detection:14903-1-AP 1:3500) with mouse brain tissue lysate 3600ug.

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  IF Staining of SH-SY5Y using 14903-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of PC-12 using 14903-1-AP

  Immunofluorescent analysis of PC-12 cells using 14903-1-AP (SNAP25 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 14903-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF: cellules SH-SY5Y, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14903-1-AP cible SNAP25 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAP25 Protéine recombinante Ag6695
• Nom complet: synaptosomal-associated protein, 25kDa
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: BC010647
• Symbole du gène: SNAP25
• Identification du gène (NCBI): 6616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Publications

Species	Application	Title
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
rat	WB	Front Cell Infect Microbiol; PMID: 28491822; Authors - Anna Piotrowska
mouse	WB	Clin Sci (Lond); Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.; Authors - Jizheng Chen
rat	WB,IF	Front Mol Neurosci; MicroRNA-210-5p Contributes to Cognitive Impairment in Early Vascular Dementia Rat Model Through Targeting Snap25.; Authors - Zhenxing Ren
mouse	WB,IF	Biomedicines; Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy.; Authors - Hugo Ramos
mouse	WB	Int Immunopharmacol; Chrysin alleviates lipopolysaccharide-induced neuron damage and behavioral deficits in mice through inhibition of Fyn; Authors - Qingchen Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (09-29-2021)

Antibody worked great and was extremely specific to SNAP-25.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neurogenin 2 iPSC Neurons Day 21

SNAP25 Antibody Western Blot validation (1:1000 dilution) in Neurogenin 2 iPSC Neurons Day 21 (Cat no:14903-1-AP)

FH

Q (Verified Customer) (03-22-2021)

An excellent SNAP25 Ab with a high titer. Very specific and clean.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: human postmortem brain lysate

SNAP25 Antibody Western Blot validation (1:5000 dilution) in human postmortem brain lysate (Cat no:14903-1-AP)

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