Anticorps Polyclonal de lapin anti-SNAP25
SNAP25 Polyclonal Antibody for ELISA
Hôte / Isotype
Goat / IgG
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 30003-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Dilution recommandée
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 2 publications below |
| IF | See 1 publications below |
Informations sur le produit
30003-1-Ig cible SNAP25 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | rat, souris |
| Hôte / Isotype | Goat / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Protéine recombinante |
| Nom complet | synaptosomal-associated protein, 25kDa |
| Masse moléculaire calculée | 23 kDa |
| Numéro d’acquisition GenBank | BC010647 |
| Symbole du gène | SNAP25 |
| Identification du gène (NCBI) | 6616 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by Botox E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
Publications
| Species | Application | Title |
|---|---|---|
Neurochem Res Intrathecal Injection of Botulinum Toxin Type A has an Analgesic Effect in Male Rats CCI Model by Inhibiting the Activation of Spinal P2X4R | ||
Free Radic Biol Med Bavachin ameliorates neuroinflammation and depressive-like behaviors in streptozotocin-induced diabetic mice through the inhibition of PKCδ |
