Anticorps Recombinant de lapin anti-SNAP25

SNAP25 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240069A1

N° de cat : 83259-1-PBS

Synonymes

240069A1, RIC 4, RIC4, RIC-4, SNAP

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Galerie de données de validation

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83259-1-RR (same clone as 83259-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83259-1-RR (SNAP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (83259-1-RR, Clone: 240069A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-12 using 83259-1-RR (same clone as 83259-1-PBS)

  1x10^6 PC-12 cells were intracellularly stained with 0.25 ug SNAP25 Recombinant antibody (83259-1-RR, Clone:240069A1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83259-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00196-1

  Cytometric bead array standard curve of MP00196-1, SNAP25 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83259-3-PBS. Detection antibody: 83259-1-PBS. Standard: Ag6695. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83259-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83259-1-PBS against Human SNAP25 was performed. The affinity constant is 2.29 nM.

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  Affinity and Kinetic Characterization of 83259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-1-RR against Human SNAP25 were performed. The affinity constant is 0.322 nM.

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Informations sur le produit

83259-1-PBS cible SNAP25 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNAP25 Protéine recombinante Ag6695
• Nom complet: synaptosomal-associated protein, 25kDa
• Masse moléculaire calculée: 23 kDa
• Numéro d’acquisition GenBank: BC010647
• Symbole du gène: SNAP25
• Identification du gène (NCBI): 6616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

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