Anticorps Polyclonal de lapin anti-SNAP25

• Phare
• Validé par KD/KO

SNAP25 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14903-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis using 14903-1-AP (same clone as 14903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 14903-1-AP (same clone as 14903-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14903-1-AP (SNAP25 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14903-1-AP (same clone as 14903-1-PBS)

  IP result of anti-SNAP25 (IP:14903-1-AP, 3ug; Detection:14903-1-AP 1:3500) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SNAP25 antibody (14903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 14903-1-AP (same clone as 14903-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (14903-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14903-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14903-1-PBS cible SNAP25 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAP25 Protéine recombinante Ag6695
• Nom complet: synaptosomal-associated protein, 25kDa
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: BC010647
• Symbole du gène: SNAP25
• Identification du gène (NCBI): 6616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by AX type E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

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