Anticorps Recombinant de lapin anti-SNAP25

SNAP25 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240069F12

N° de cat : 83259-5-RR

Synonymes

SNAP 25, SNAP, RIC-4, RIC4, RIC 4

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Galerie de données de validation

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  WB analysis using 83259-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83259-5-RR (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-12 using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of SH-SY5Y using 83259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNAP25 antibody (83259-5-RR, Clone: 240069F12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83259-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83259-5-RR against Human SNAP25 were performed. The affinity constant is 0.388 nM.

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Applications testées

• Résultats positifs en WB: cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules PC-12, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83259-5-RR cible SNAP25 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNAP25 Protéine recombinante Ag6695
• Nom complet: synaptosomal-associated protein, 25kDa
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC010647
• Symbole du gène: SNAP25
• Identification du gène (NCBI): 6616
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

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