Anticorps Polyclonal de lapin anti-SND1

• Phare
• Validé par KD/KO

SND1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, RIP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10760-1-AP

Synonymes

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

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Galerie de données de validation

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  WB analysis using 10760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10760-1-AP (SND1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10760-1-AP

  WB result of SND1 antibody (10760-1-AP, 1:4000) with si-Control and si-SND1 transfected Jurkat cells.

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  IHC staining of human breast hyperplasia using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10760-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10760-1-AP (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10760-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10760-1-AP (SND1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu d'hyperplasie mammaire humain, tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

10760-1-AP cible SND1 dans les applications de WB, RIP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SND1 Protéine recombinante Ag1200
• Nom complet: staphylococcal nuclease and tudor domain containing 1
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC017180
• Symbole du gène: SND1
• Identification du gène (NCBI): 27044
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. This is a rabbit polyclonal antibody raised against near the C-terminus of human SND1.

Publications

Species	Application	Title
human	IF	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	RIP	Cell Res; Predicting dynamic cellular protein-RNA interactions by deep learning using in vivo RNA structures.; Authors - Lei Sun
human	WB,ChIP,RIP	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez ; KD Validated
mouse	WB,IHC	J Proteome Res; Novel breast cancer metastasis-associated proteins.; Authors - Ho Jiapei J
	WB	J Cell Physiol; The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.; Authors - Sen Zhang