Anticorps Monoclonal anti-SND1

• Phare
• Validé par KD/KO

SND1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

1A6A4

N° de cat : 60265-1-Ig

Synonymes

100 kDa coactivator, EBNA2 coactivator p100, p100, p100 co activator, SND1, TDRD11

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Galerie de données de validation

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  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60265-1-Ig

  WB result of SND1 antibody (60265-1-Ig, 1:60,000) with si-Control and si-SND1 transfected Jurkat cells.

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  WB analysis using 60265-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60265-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60265-1-Ig (SND1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60265-1-Ig

  IP Result of anti-SND1 (IP:60265-1-Ig, 5ug; Detection:60265-1-Ig 1:500) with HeLa cells lysate 1400ug.

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  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60265-1-Ig (SND1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast hyperplasia using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60265-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60265-1-Ig (SND1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60265-1-Ig(SND1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of HepG2 using 60265-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SND1 antibody (60265-1-Ig, Clone: 1A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu d'hyperplasie mammaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60265-1-Ig cible SND1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SND1 Protéine recombinante Ag1200
• Nom complet: staphylococcal nuclease and tudor domain containing 1
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC017180
• Symbole du gène: SND1
• Identification du gène (NCBI): 27044
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors.

Publications

Species	Application	Title
human	WB	Cell; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9; Authors - Nora Schmidt
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	IF, ELISA	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Elife; The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus.; Authors - Belinda Baquero-Perez ; KD Validated
	IF	J Cell Physiol; The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs.; Authors - Sen Zhang
human	WB,IF,CoIP	J Exp Clin Cancer Res; Circ_0004087 interaction with SND1 promotes docetaxel resistance in prostate cancer by boosting the mitosis error correction mechanism.; Authors - Liang Chen