Anticorps Monoclonal anti-SNF8

SNF8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C10F2

N° de cat : 67696-1-PBS

Synonymes

2C10F2, Dot3, EAP30, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22

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Galerie de données de validation

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  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67696-1-Ig (same clone as 67696-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67696-1-Ig (same clone as 67696-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67696-1-Ig (SNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67696-1-Ig (SNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67696-1-Ig (same clone as 67696-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNF8 antibody (67696-1-Ig, Clone: 2C10F2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67696-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67696-1-PBS cible SNF8 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNF8 Protéine recombinante Ag30384
• Nom complet: SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
• Masse moléculaire calculée: 258 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC008976
• Symbole du gène: SNF8
• Identification du gène (NCBI): 11267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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