Anticorps Recombinant de lapin anti-SNIP1

SNIP1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230287D11

N° de cat : 82962-1-PBS

Synonymes

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

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Galerie de données de validation

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  WB analysis using 82962-1-RR (same clone as 82962-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82962-1-RR (same clone as 82962-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82962-1-RR (same clone as 82962-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82962-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82962-1-PBS cible SNIP1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNIP1 Protéine recombinante Ag33543
• Nom complet: Smad nuclear interacting protein 1
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC027040
• Symbole du gène: SNIP1
• Identification du gène (NCBI): 79753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

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