Anticorps Recombinant de lapin anti-SNRPA

SNRPA Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251055G1

N° de cat : 86259-3-RR

Synonymes

U1 A, U1 snRNP A, U1A, U1-A

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Galerie de données de validation

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  WB analysis using 86259-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86259-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86259-3-RR (SNRPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86259-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNRPA antibody (86259-3-RR, Clone: 251055G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86259-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86259-3-RR against Human SNRPA were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, tissu cérébral de souris, tissu de thymus de rat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86259-3-RR cible SNRPA dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNRPA Protéine recombinante Ag0283
• Nom complet: small nuclear ribonucleoprotein polypeptide A
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC000405
• Symbole du gène: SNRPA
• Identification du gène (NCBI): 6626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

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