Anticorps Monoclonal anti-SNRPB2

SNRPB2 Monoclonal Antibody for Indirect ELISA, WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

1D11E9

N° de cat : 68095-1-PBS

Synonymes

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

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Galerie de données de validation

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  WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68095-1-PBS cible SNRPB2 dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNRPB2 Protéine recombinante Ag5540
• Nom complet: small nuclear ribonucleoprotein polypeptide B''
• Masse moléculaire calculée: 225 aa, 26, 5 kDa
• Poids moléculaire observé: 25-31 kDa
• Numéro d’acquisition GenBank: BC036737
• Symbole du gène: SNRPB2
• Identification du gène (NCBI): 6629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

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