Anticorps Monoclonal anti-SNX12

SNX12 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C6C8

N° de cat : 68260-1-PBS

Synonymes

Sorting nexin-12, 2C6C8

---

Galerie de données de validation

• 

  WB analysis using 68260-1-Ig (same clone as 68260-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68260-1-Ig (SNX12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68260-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68260-1-Ig (same clone as 68260-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (68260-1-Ig, Clone: 2C6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68260-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68260-1-PBS cible SNX12 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNX12 Protéine recombinante Ag3014
• Nom complet: sorting nexin 12
• Masse moléculaire calculée: 172 aa, 20 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC020559
• Symbole du gène: SNX12
• Identification du gène (NCBI): 29934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

{{ptg:RelatedPrimaryAntibodies}}