Anticorps Monoclonal anti-SNX15

SNX15 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10B7

N° de cat : 68239-1-PBS

Synonymes

Sorting nexin-15, sorting nexin 15, HSAF001435, 1A10B7

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Galerie de données de validation

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  WB analysis using 68239-1-Ig (same clone as 68239-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68239-1-Ig (same clone as 68239-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (68239-1-Ig, Clone: 1A10B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68239-1-PBS cible SNX15 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNX15 Protéine recombinante Ag8839
• Nom complet: sorting nexin 15
• Masse moléculaire calculée: 342 aa, 38 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC009897
• Symbole du gène: SNX15
• Identification du gène (NCBI): 29907
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

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