Anticorps Polyclonal de lapin anti-SNX17

• Phare
• Validé par KD/KO

SNX17 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 10275-1-AP

Synonymes

KIAA0064, SNX17, sorting nexin 17

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Galerie de données de validation

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  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 10275-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10275-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10275-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10275-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10275-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10275-1-AP

  IP Result of anti-SNX17 (IP:10275-1-AP, 4ug; Detection:10275-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris, tissu cérébral humain, tissu cutané de souris, tissu de cervelet de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 18 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

10275-1-AP cible SNX17 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX17 Protéine recombinante Ag0355
• Nom complet: sorting nexin 17
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002524
• Symbole du gène: SNX17
• Identification du gène (NCBI): 9784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
mouse	WB	Nat Cell Biol; Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.; Authors - B?ttcher Ralph Thomas RT ; KD Validated
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Proc Natl Acad Sci U S A; LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.; Authors - Björn Schorch ; KD Validated

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