Anticorps Recombinant de lapin anti-SNX18

SNX18 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241537D11

N° de cat : 84215-4-RR

Synonymes

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11

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Galerie de données de validation

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  WB analysis using 84215-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 84215-4-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84215-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-4-RR, Clone: 241537D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84215-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84215-4-RR against Human SNX18 were performed. The affinity constant is 0.378 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84215-4-RR cible SNX18 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SNX18 Protéine recombinante Ag16645
• Nom complet: sorting nexin 18
• Masse moléculaire calculée: 628 aa, 69 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC060791
• Symbole du gène: SNX18
• Identification du gène (NCBI): 112574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

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