Anticorps Polyclonal de lapin anti-SNX25

• Phare
• Validé par KD/KO

SNX25 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13294-1-AP

Synonymes

Sorting nexin-25, sorting nexin 25, SBBI31, MSTP043

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Galerie de données de validation

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  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13294-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13294-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13294-1-AP

  IP result of anti-SNX25 (IP:13294-1-AP, 4ug; Detection:13294-1-AP 1:2000) with Raji cells lysate 1600 ug.

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  IF Staining of HeLa using 13294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX25 antibody (13294-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: EL-4 cells, cellules C6, cellules Jurkat, cellules Raji, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de souris
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13294-1-AP cible SNX25 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX25 Protéine recombinante Ag3995
• Nom complet: sorting nexin 25
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 98-105 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC029868
• Symbole du gène: SNX25
• Identification du gène (NCBI): 83891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.

Publications

Species	Application	Title
mouse	IHC	Brain Struct Funct; Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking.; Authors - Shoko Takemura
mouse	WB	PLoS One; SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.; Authors - Kazuya Nishimura ; KD Validated
mouse	WB	Neurosci Lett; Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus.; Authors - Shoko Takemura
mouse	WB,IF	Nat Immunol; Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway; Authors - Tatsuhide Tanaka
human	WB,CoIP	Redox Biol; Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes; Authors - Yulong Zhang
mouse	WB	Sci Rep; Dermal macrophages control tactile perception under physiological conditions via NGF signaling; Authors - Tatsuhide Tanaka

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