Anticorps Polyclonal de lapin anti-SNX25

• Phare
• Validé par KD/KO

SNX25 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13294-1-AP

Synonymes

MSTP043, SBBI31, SNX25, sorting nexin 25

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Galerie de données de validation

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  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13294-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13294-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13294-1-AP (SNX25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: EL-4 cells, cellules C6, cellules Jurkat, cellules Raji, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13294-1-AP cible SNX25 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX25 Protéine recombinante Ag3995
• Nom complet: sorting nexin 25
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 98-105 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC029868
• Symbole du gène: SNX25
• Identification du gène (NCBI): 83891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNXs (sorting nexin), a family of proteins playing roles in cargo sorting and signaling from compartments within the endocytic network, regulate traffic of membrane proteins including TGF-β receptors. Sorting Nexin 25 (SNX25) has been recently proposed to modulate TGF-β signaling through endosomal sorting of TGF-β receptors for lysosomal degradation.This antibody can recognize two isoforms: 98-105 kDa and 65 kDa.

Publications

Species	Application	Title
mouse	IHC	Brain Struct Funct; Neural expression of sorting nexin 25 and its regulation of tyrosine receptor kinase B trafficking.; Authors - Shoko Takemura
mouse	WB	PLoS One; SNX25 regulates proinflammatory cytokine expression via the NF-κB signal in macrophages.; Authors - Kazuya Nishimura ; KD Validated
mouse	WB	Neurosci Lett; Circadian rhythms of sorting nexin 25 in the mouse suprachiasmatic nucleus.; Authors - Shoko Takemura
mouse	WB,IF	Nat Immunol; Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway; Authors - Tatsuhide Tanaka