Anticorps Polyclonal de lapin anti-SNX27

SNX27 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16329-1-AP

Synonymes

KIAA0488, MRT1, MY014, SNX27, Sorting nexin 27, sorting nexin family member 27

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Galerie de données de validation

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  WB analysis of Jurkat using 16329-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16329-1-AP (SNX27 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16329-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16329-1-AP (SNX27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

16329-1-AP cible SNX27 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX27 Protéine recombinante Ag9447
• Nom complet: sorting nexin family member 27
• Masse moléculaire calculée: 61 kDa, 60 kDa, 52 kDa, 28 kDa
• Poids moléculaire observé: 52-60 kDa
• Numéro d’acquisition GenBank: BC012184
• Symbole du gène: SNX27
• Identification du gène (NCBI): 81609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to the synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).

Publications

Species	Application	Title
human	WB	J Cell Biol; Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.; Authors - Boris Simonetti
human	WB	Cells; Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells; Authors - Jiahe Lu
human	WB	bioRxiv; Five inhibitory receptors display distinct vesicular distributions in T cells; Authors - Jiahe Lu
human	WB	Cell Rep; Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.; Authors - Xuan Ling Hilary Yong
human,mouse	WB	Endocrinol Diabetes Metab; Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle; Authors - Rachel Livingstone

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