Anticorps Polyclonal de lapin anti-SNX3

• Phare
• Validé par KD/KO

SNX3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 10772-1-AP

Synonymes

Grd19, MCOPS8, Protein SDP3, SDP3, sorting nexin 3

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Galerie de données de validation

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  WB analysis of COLO 320 using 10772-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10772-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10772-1-AP (SNX3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10772-1-AP (SNX3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 10772-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (10772-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 10772-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX3 antibody (10772-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules A549, cellules HeLa
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

10772-1-AP cible SNX3 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX3 Protéine recombinante Ag1160
• Nom complet: sorting nexin 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC008444
• Symbole du gène: SNX3
• Identification du gène (NCBI): 8724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.; Authors - Fergal O'Farrell ; KD Validated
mouse	WB,IHC,IF,CoIP	Acta Pharm Sin B; Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis; Authors - Wenjing Yu
human	WB,IF	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough ; KD Validated
mouse	WB	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
rat,mouse	WB,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu ; KO Validated
human	WB,IF	Oncogene; EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.; Authors - Esra Cicek ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rafeeq (Verified Customer) (01-30-2019)

I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs

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