Anticorps Polyclonal de lapin anti-SNX5

• Phare
• Validé par KD/KO

SNX5 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 17918-1-AP

Synonymes

Sorting nexin-5

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Galerie de données de validation

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  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17918-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 17918-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17918-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 17918-1-AP

  IP result of anti-SNX5 (IP:17918-1-AP, 4ug; Detection:17918-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17918-1-AP (SNX5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules U2OS, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17918-1-AP cible SNX5 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNX5 Protéine recombinante Ag12330
• Nom complet: sorting nexin 5
• Masse moléculaire calculée: 404 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC093623
• Symbole du gène: SNX5
• Identification du gène (NCBI): 27131
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough
mouse	WB	Proc Natl Acad Sci U S A; Retromer dysfunction in amyotrophic lateral sclerosis.; Authors - Eduardo J Pérez-Torres
human	WB	J Cell Biol; Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.; Authors - Boris Simonetti ; KO Validated
rat	WB	Oxid Med Cell Longev; Sorting Nexin 5 Plays an Important Role in Promoting Ferroptosis in Parkinson's Disease.; Authors - Zifeng Huang ; KD Validated
human	WB	Cell Rep; The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking; Authors - Khavong Pha

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