Anticorps Monoclonal anti-SNX9

SNX9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B11C9

N° de cat : 68255-1-PBS

Synonymes

MST155, MSTP155, Protein SDP1, SDP1, SH3PX1, SH3PXD3A, SNX9, sorting nexin 9, WISP

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Galerie de données de validation

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  WB analysis using 68255-1-Ig (same clone as 68255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68255-1-Ig (SNX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68255-1-Ig (same clone as 68255-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68255-1-Ig (SNX9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68255-1-Ig (same clone as 68255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SNX9 antibody (68255-1-Ig, Clone: 1B11C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68255-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68255-1-PBS cible SNX9 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SNX9 Protéine recombinante Ag8446
• Nom complet: sorting nexin 9
• Masse moléculaire calculée: 595 aa, 67 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC005022
• Symbole du gène: SNX9
• Identification du gène (NCBI): 51429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

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