Anticorps Polyclonal de lapin anti-SORBS1

• Phare
• Validé par KD/KO

SORBS1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13854-1-AP

Synonymes

CAP, c-Cbl-associated protein, SH3D5, SORB1, Sorbin and SH3 domain-containing protein 1

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 13854-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13854-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13854-1-AP

  IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug.

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  IHC staining of human colon using 13854-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13854-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules 3T3-L1, cellules HEK-293, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13854-1-AP cible SORBS1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORBS1 Protéine recombinante Ag4828
• Nom complet: sorbin and SH3 domain containing 1
• Masse moléculaire calculée: 1292 aa, 142 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC042612
• Symbole du gène: SORBS1
• Identification du gène (NCBI): 10580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The sorbin homology (SoHo) family of adapter and scaffold proteins consists of three proteins: CAP, also known as Sorbin and SH3 domain containing 1 (Sorbs1), ArgBP2, also known as Sorbs2, and Vinexin, also known as Sorbs3. All Sorbs proteins are highly expressed in heart tissue, skeletal muscle, adipose tissue, and cells of the immune system, functioning as SH3-domain-mediated adaptors of scaffolding molecules(PMID: 32738595). SORBS1 can bind to signaling molecules (e.g., c-Cbl, c-Abl, and insulin reporter) to regulate glucose transport, transcription activity, and insulin signaling(PMID: 27791200).

Publications

Species	Application	Title
mouse,human	WB,IHC	Front Cell Dev Biol; Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.; Authors - Yiyang Xu ; KD Validated
mouse	IF	Front Cell Neurosci; Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.; Authors - Anchel González-Barriga
canine	IF	PLoS One; Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells.; Authors - Karin Fredriksson-Lidman
mouse,human	WB	Sci Rep; STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation; Authors - Yuichi Sekine
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono

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