Anticorps Polyclonal de lapin anti-SORBS1

• Phare
• Validé par KD/KO

SORBS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13854-1-PBS

Synonymes

CAP, c-Cbl-associated protein, SH3D5, SORB1, Sorbin and SH3 domain-containing protein 1

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Galerie de données de validation

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  WB analysis of 3T3-L1 using 13854-1-AP (same clone as 13854-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13854-1-AP (same clone as 13854-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13854-1-AP (same clone as 13854-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13854-1-AP (same clone as 13854-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 13854-1-AP (same clone as 13854-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 13854-1-AP (same clone as 13854-1-PBS)

  IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13854-1-AP (same clone as 13854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 13854-1-AP (same clone as 13854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13854-1-PBS cible SORBS1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORBS1 Protéine recombinante Ag4828
• Nom complet: sorbin and SH3 domain containing 1
• Masse moléculaire calculée: 1292 aa, 142 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC042612
• Symbole du gène: SORBS1
• Identification du gène (NCBI): 10580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The sorbin homology (SoHo) family of adapter and scaffold proteins consists of three proteins: CAP, also known as Sorbin and SH3 domain containing 1 (Sorbs1), ArgBP2, also known as Sorbs2, and Vinexin, also known as Sorbs3. All Sorbs proteins are highly expressed in heart tissue, skeletal muscle, adipose tissue, and cells of the immune system, functioning as SH3-domain-mediated adaptors of scaffolding molecules(PMID: 32738595). SORBS1 can bind to signaling molecules (e.g., c-Cbl, c-Abl, and insulin reporter) to regulate glucose transport, transcription activity, and insulin signaling(PMID: 27791200).

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