Anticorps Polyclonal de lapin anti-SORBS2

• Phare
• Validé par KD/KO

SORBS2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24643-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 24643-1-AP (same clone as 24643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 24643-1-AP (same clone as 24643-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 24643-1-AP (same clone as 24643-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 24643-1-AP (same clone as 24643-1-PBS)

  IP result of anti-SORBS2 (IP:24643-1-AP, 4ug; Detection:24643-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 24643-1-AP (same clone as 24643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 24643-1-AP (same clone as 24643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24643-1-PBS cible SORBS2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SORBS2 Protéine recombinante Ag20041
• Nom complet: sorbin and SH3 domain containing 2
• Masse moléculaire calculée: 1100 aa, 124 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC011883
• Symbole du gène: SORBS2
• Identification du gène (NCBI): 8470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

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