Anticorps Recombinant de lapin anti-SORBS2

SORBS2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240023A8

N° de cat : 83189-3-PBS

Synonymes

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

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Galerie de données de validation

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  WB analysis using 83189-3-RR (same clone as 83189-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83189-3-RR (SORBS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83189-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83189-3-RR (same clone as 83189-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SORBS2 antibody (83189-3-RR, Clone: 240023A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83189-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83189-3-RR (same clone as 83189-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SORBS2 Recombinant antibody (83189-3-RR, Clone:240023A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83189-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83189-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83189-3-RR against Human SORBS2 were performed. The affinity constant is 0.433 nM.

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Informations sur le produit

83189-3-PBS cible SORBS2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SORBS2 Protéine recombinante Ag20041
• Nom complet: sorbin and SH3 domain containing 2
• Masse moléculaire calculée: 1100 aa, 124 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC011883
• Symbole du gène: SORBS2
• Identification du gène (NCBI): 8470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

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