Anticorps Recombinant de lapin anti-SORBS2

SORBS2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240023A8

N° de cat : 83189-3-RR

Synonymes

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

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Galerie de données de validation

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  WB analysis using 83189-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83189-3-RR (SORBS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83189-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SORBS2 antibody (83189-3-RR, Clone: 240023A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U2OS using 83189-3-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SORBS2 Recombinant antibody (83189-3-RR, Clone:240023A8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83189-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83189-3-RR against Human SORBS2 were performed. The affinity constant is 0.433 nM.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HuH-7
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

83189-3-RR cible SORBS2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SORBS2 Protéine recombinante Ag20041
• Nom complet: sorbin and SH3 domain containing 2
• Masse moléculaire calculée: 1100 aa, 124 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC011883
• Symbole du gène: SORBS2
• Identification du gène (NCBI): 8470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

Publications

Species	Application	Title
human,mouse	WB,IF	Nature; Selective inhibition of stromal mechanosensing suppresses cardiac fibrosis; Authors - Sangkyun Cho

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