Anticorps Recombinant de lapin anti-SORBS2

SORBS2 Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240023A8

N° de cat : CL488-83189-3

Synonymes

Sorbin and SH3 domain-containing protein 2, ArgBP2, Arg-binding protein 2, Arg/Abl-interacting protein 2, 240023A8

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Galerie de données de validation

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  IF Staining of U2OS using CL488-83189-3

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 SORBS2 antibody (CL488-83189-3, Clone: 240023A8 ) at dilution of 1:200.

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  FC experiment of U2OS using CL488-83189-3

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SORBS2 Recombinant antibody (CL488-83189-3, Clone:240023A8)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-83189-3 cible SORBS2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SORBS2 Protéine recombinante Ag20041
• Nom complet: sorbin and SH3 domain containing 2
• Masse moléculaire calculée: 1100 aa, 124 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC011883
• Symbole du gène: SORBS2
• Identification du gène (NCBI): 8470
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

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