Anticorps Polyclonal de lapin anti-SORCS2

SORCS2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 55063-1-AP

Synonymes

KIAA1329, SorCS2 104 kDa chain, SorCS2 122 kDa chain, SorCS2 18 kDa chain, VPS10 domain-containing receptor SorCS2

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Galerie de données de validation

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  WB analysis of Jurkat using 55063-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55063-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55063-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HaCaT using 55063-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using SORCS2 antibody (55063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HaCaT,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

55063-1-AP cible SORCS2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sortilin-related VPS10 domain containing receptor 2
• Masse moléculaire calculée: 128 kDa
• Poids moléculaire observé: 128-140 kDa
• Numéro d’acquisition GenBank: NM_020777
• Symbole du gène: SORCS2
• Identification du gène (NCBI): 57537
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SORCS2, also named as KIAA1329, is one of the VPS10 domain-containing receptors. It contains 6 BNR repeats and 1 PKD domain.. The antibody is specific to SORCS2.

Publications

Species	Application	Title
rat	WB	Cytojournal; Astragalus polysaccharide attenuates retinal ischemia reperfusion-induced microglial activation through sortilin-related vacuolar protein sorting 10 domain containing receptor 2/laminin subunit alpha 1 upregulation; Authors - Juanjuan Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juan Pablo (Verified Customer) (11-29-2018)

I tried with different lysis buffer. I tried blocking with 5% milk or 2% BSA. Never worked for me. Didn't work for immunohistochemistry either dilution 1:1000 and 1:100.

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1-500
• Cell Tissue Type: Postnatal cerebellum, from P2 to P14

SORCS2 Antibody Western Blot,Immunohistochemistry, validation (1-500 dilution) in Postnatal cerebellum, from P2 to P14 (Cat no:55063-1-AP)

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