Anticorps Polyclonal de lapin anti-SOX10

• Phare
• Validé par KD/KO

SOX10 Polyclonal Antibody for WB, IHC, IF-Fro, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(30)

N° de cat : 10422-1-AP

Synonymes

DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10, Transcription factor SOX-10

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Galerie de données de validation

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  WB analysis of mouse brain using 10422-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453 using 10422-1-AP

  WB result of SOX10 antibody (10422-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX10 transfected MDA-MB-453 cells.

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  WB analysis of SH-SY5Y using 10422-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10422-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10422-1-AP (SOX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse colon using 10422-1-AP

  IP result of anti-SOX10 (IP:10422-1-AP, 4ug; Detection:10422-1-AP 1:2000) with mouse colon tissue lysate 4000 ug.

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  IHC staining of mouse brain using 10422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10422-1-AP (SOX10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using SOX10 antibody (10422-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of C6 using 10422-1-AP

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (10422-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu d'intestin grêle de souris
• Résultats positifs en IP: tissu de côlon de souris,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 19 publications below
• CoIP: See 1 publications below

Informations sur le produit

10422-1-AP cible SOX10 dans les applications de WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX10 Protéine recombinante Ag0673
• Nom complet: SRY (sex determining region Y)-box 10
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC002824
• Symbole du gène: SOX10
• Identification du gène (NCBI): 6663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Publications

Species	Application	Title
mouse	IHC	Nature; Regulation of intestinal immunity and tissue repair by enteric glia; Authors - Fränze Progatzky
mouse	IF	Neuron; Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments; Authors - Jie Xiang
rat,mouse	WB,IF,CoIP	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	IF	Front Immunol; Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas; Authors - Gelei Xiao
mouse	WB	J Cereb Blood Flow Metab; The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia; Authors - Celeste Yin-Chieh Wu
rat	WB,IF	J Neurosci; H3K27 Trimethylation-Mediated Downregulation of miR-216a-3p in Sensory Neurons Regulates Neuropathic Pain Behaviors via Targeting STIM1; Authors - Yufang Sun

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