Anticorps Monoclonal anti-SOX10

• Phare
• Validé par KD/KO

SOX10 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(16)

CloneNo.

1D2C8

Avis (2)

N° de cat : 66786-1-Ig

Synonymes

1D2C8, DOM, PCWH, SRY-box transcription factor 9, Transcription factor SOX 10

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Galerie de données de validation

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  WB analysis of A375 using 66786-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66786-1-Ig (SOX10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453 using 66786-1-Ig

  WB result of SOX10 antibody (66786-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX10 transfected MDA-MB-453 cells.

• 

  IHC staining of human malignant melanoma using 66786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of rat cerebellum using 66786-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 66786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of mouse brain using 66786-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66786-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66786-1-Ig (SOX10 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of rat brain using 66786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 66786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of A375 using 66786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using SOX10 antibody (66786-1-Ig, Clone: 1D2C8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  FC experiment of C6 using 66786-1-Ig

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human SOX10 (66786-1-Ig, Clone:1D2C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A375, MDA-MB-453 cells
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu cérébral de souris, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF-Fro: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Informations sur le produit

66786-1-Ig cible SOX10 dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, poulet, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOX10 Protéine recombinante Ag23191
• Nom complet: SRY (sex determining region Y)-box 10
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC002824
• Symbole du gène: SOX10
• Identification du gène (NCBI): 6663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Publications

Species	Application	Title
mouse	IF	Elife; Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve.; Authors - Sonia Taïb
chicken	IF	Front Physiol; Cadherin-11 Is Required for Neural Crest Specification and Survival.; Authors - Subrajaa Manohar
rat	IF	Physiol Behav; Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria.; Authors - Carolina R Cawthon
	IF	J Neurosci Methods; Characterization of the GfaABC1D promoter to selectively target astrocytes in the rhesus macaque brain.; Authors - Kate S Heffernan
rabbit	WB	Anim Biosci; Exosomal miR-222-3p derived from dermal papilla cells inhibits melanogenesis in melanocytes by targeting SOX10 in rabbits; Authors - Yang Chen
rabbit	IHC	Res Sq; Pleiotrophin-Neuregulin1 promote axon regeneration and sorting in conduit repair of critical nerve gap injuries; Authors - Mario RomeroOrtega

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sonia (Verified Customer) (07-24-2019)

Works fine in longitudinal section of mouse sciatic nerve

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: sciatic nerve

SOX10 Antibody Immunofluorescence, validation (1/200 dilution) in sciatic nerve (Cat no:66786-1-Ig)

FH

Crystal (Verified Customer) (02-19-2019)

Works great in chicken embryos! We used 1:500-1:1000 dilution for the primary and made sure to wash it extensively between primary and secondary.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Chicken Embryo

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