Anticorps Monoclonal anti-SOX10

SOX10 Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

1D2C8

N° de cat : CL594-66786

Synonymes

Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH, DOM

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Galerie de données de validation

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  IF Staining of mouse brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594-conjugated SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:100.

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  IF Staining of mouse brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200. DAPI (blue).

• 

  IF Staining of rat brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

• 

  IF Staining of rat brain using CL594-66786

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®594 SOX10 antibody (CL594-66786, Clone: 1D2C8 ) at dilution of 1:200.

• 

  FC experiment of C6 using CL594-66786

  1X10^6 C6 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SOX10 (CL594-66786, Clone:1D2C8) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en FC (Intra): cellules C6,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-66786 cible SOX10 dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SOX10 Protéine recombinante Ag23191
• Nom complet: SRY (sex determining region Y)-box 10
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: BC002824
• Symbole du gène: SOX10
• Identification du gène (NCBI): 6663
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Publications

Species	Application	Title
mouse	IF	Nat Commun; Suppressing recurrence in Sonic Hedgehog subgroup medulloblastoma using the OLIG2 inhibitor CT-179; Authors - Yuchen Li

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