Anticorps Polyclonal de lapin anti-SOX17

• Phare
• Validé par KD/KO

SOX17 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(19)

N° de cat : 24903-1-AP

Synonymes

Transcription factor SOX-17, Transcription factor SOX 17

---

Galerie de données de validation

• 

  WB analysis using 24903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24903-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules C6, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 15 publications below

Informations sur le produit

24903-1-AP cible SOX17 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SOX17 Protéine recombinante Ag19157
• Nom complet: SRY (sex determining region Y)-box 17
• Masse moléculaire calculée: 414 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC111365
• Symbole du gène: SOX17
• Identification du gène (NCBI): 64321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor SOX 17 (SOX17) also named as SRY box 17 is a 414 amino acid protein, which contains one Sox C-terminal domain and one HMG box DNA-binding domain. SOX17 as a transcription regulator binds target promoter DNA and bend the DNA via WNT3A. SOX17 is a marker of endodermal cells and a transcriptional regulator containing a DNA binding domain called the HMG box. In mouse embryos, SOX17 plays critical roles in the growth and differentiation of definitive endodermal cells (PMID: 11973269), the remodeling of endothelial cells (PMID: 16895970), hindgut endoderm expansion with localization of primordial germ cells (PMID: 19371732), and gallbladder/bile duct formation (PMID: 19913509).

Publications

Species	Application	Title
human	IF	Mater Today Bio; Nanofiber-microwell cell culture system for spatially patterned differentiation of pluripotent stem cells in 3D; Authors - Youyi Tai
mouse	WB	Stem Cell Reports; Phosphorylation of Threonine343 Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency.; Authors - Xianmixinuer Abulaiti
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun
human	IF	Stem Cell Res; Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA).; Authors - Jiajia Yang
human	IF	Stem Cell Res; Generation of a Friedreich's Ataxia patient-derived iPSC line USFi001-A.; Authors - Mariana Burgos Angulo
human	IF	Stem Cell Res; Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C).; Authors - Jiajia Yang

{{ptg:RelatedPrimaryAntibodies}}